Sobrevivência de Salmonella enterica às condições gástricas simuladas in vitro: papel dos fatores de adesão e resistência antibacteriana

Jhennifer Arruda Schmiedt; Emanoelli Aparecida Rodrigues dos  Santos; Leonardo Ereno Tadielo; Tatiane Barbosa Coitinho; Carolina Dias Rodrigues; Luiz Gustavo Bach; Taís Luana Pinto; Juliano Gonçalves Pereira; Vanessa Mendonça Soares; Thiago Henrique Bellé; Vinicius Cunha Barcellos; Luciano dos Santos Bersot

doi:10.18593/evid.32846

Autores

Jhennifer Arruda Schmiedt Universidade Federal do Paraná
Emanoelli Aparecida Rodrigues dos Santos Universidade Estadual Paulista https://orcid.org/0000-0001-6011-3232
Leonardo Ereno Tadielo Universidade Estadual Paulista
Tatiane Barbosa Coitinho Universidade Federal do Paraná
Carolina Dias Rodrigues Universidade Federal do Paraná
Luiz Gustavo Bach Universidade Federal do Paraná https://orcid.org/0000-0002-0913-8538
Taís Luana Pinto Universidade Federal do Paraná
Juliano Gonçalves Pereira Universidade Estadual Paulista
Vanessa Mendonça Soares Universidade Estadual Paulista
Thiago Henrique Bellé Universidade Federal do Paraná https://orcid.org/0000-0002-9462-6666
Vinicius Cunha Barcellos Universidade Federal do Paraná https://orcid.org/0000-0002-9404-3460
Luciano dos Santos Bersot Universidade Federal do Paraná https://orcid.org/0000-0001-7013-5574

DOI:

https://doi.org/10.18593/evid.32846

Palavras-chave:

Microrganismos resistentes, Beta-lactamases, Biofilme, Tolerância gastrointestinal, Cortes cárneos, Frango de corte

Resumo

O estudo tem como objetivo verificar a capacidade de adesão e formação de biofilme de isolados de Salmonella spp. produtores de enzimas beta-lactamases de espectro estendido (ESBL), bem como a resistência de isolados produtores ou não de ESBL, frente às condições gástricas e intestinais simuladas in vitro. Foram utilizados 18 isolados de Salmonella spp. obtidos de cortes de frangos congelados produzidos no Estado do Paraná, Brasil. Os isolados produtores de ESBL foram avaliados de forma fenotípica e genotípica quanto a capacidade de adesão e formação de biofilme em lâminas de polipropileno. Além disso, todos os isolados foram submetidos a avaliação in vitro de resistência às condições gástricas e intestinais e após as simulações, realizadas contagens das células sobreviventes. Com relação à adesão e formação de biofilme, 13 isolados apresentaram essa capacidade. Quanto a tolerância às condições gástricas e intestinais, a quantificação de Salmonella spp. diminuiu (p<0,05) conforme a passagem do trato gastrointestinal. Em relação a comparação de isolados produtores e não produtores de ESBL, não houve diferença (p>0,05) na taxa de sobrevivência total. Os resultados demonstram que, apesar da diminuição da contagem de microrganismos conforme a passagem simulada pelos sucos gástricos e intestinais, a taxa de sobrevivência média total dos isolados de Salmonella spp. foi alta, o que permite inferir que os isolados possuem a capacidade de sobreviver às condições gastrointestinais simuladas, e ainda sim permaneceram em número capaz de causar infecção.

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