Sobrevivência de Salmonella enterica às condições gástricas simuladas in vitro: papel dos fatores de adesão e resistência antibacteriana

Jhennifer Arruda Schmiedt; Emanoelli Aparecida Rodrigues dos  Santos; Leonardo Ereno Tadielo; Tatiane Barbosa Coitinho; Carolina Dias Rodrigues; Luiz Gustavo Bach; Taís Luana Pinto; Juliano Gonçalves Pereira; Vanessa Mendonça Soares; Thiago Henrique Bellé; Vinicius Cunha Barcellos; Luciano dos Santos Bersot

doi:10.18593/evid.32846

Authors

Jhennifer Arruda Schmiedt Universidade Federal do Paraná
Emanoelli Aparecida Rodrigues dos Santos Universidade Estadual Paulista https://orcid.org/0000-0001-6011-3232
Leonardo Ereno Tadielo Universidade Estadual Paulista
Tatiane Barbosa Coitinho Universidade Federal do Paraná
Carolina Dias Rodrigues Universidade Federal do Paraná
Luiz Gustavo Bach Universidade Federal do Paraná https://orcid.org/0000-0002-0913-8538
Taís Luana Pinto Universidade Federal do Paraná
Juliano Gonçalves Pereira Universidade Estadual Paulista
Vanessa Mendonça Soares Universidade Estadual Paulista
Thiago Henrique Bellé Universidade Federal do Paraná https://orcid.org/0000-0002-9462-6666
Vinicius Cunha Barcellos Universidade Federal do Paraná https://orcid.org/0000-0002-9404-3460
Luciano dos Santos Bersot Universidade Federal do Paraná https://orcid.org/0000-0001-7013-5574

DOI:

https://doi.org/10.18593/evid.32846

Keywords:

Resistant microrganisms, Beta-lactamases, Biofilm, Gastrointestinal tolerance, Meat cuts, Broiler chickens

Abstract

The study aims to assess the adhesion and biofilm formation capacity of Salmonella spp. isolates producing extended-spectrum beta-lactamases (ESBL) and the resistance of ESBL-producing and non-producing isolates to in vitro simulated gastric and intestinal conditions. Eighteen Salmonella spp. isolates were obtained from frozen chicken cuts produced in the State of Paraná, Brazil. ESBL-producing isolates were evaluated phenotypically and genotypically for their adhesion and biofilm formation capacity on polypropylene coupons. Furthermore, all isolates underwent in vitro assessment of resistance to gastric and intestinal conditions, and cell counts were conducted after the simulations. Regarding adhesion and biofilm formation, 13 isolates demonstrated this capability. Concerning tolerance to gastric and intestinal conditions, the quantification of Salmonella spp. decreased (p<0.05) as they passed through the gastrointestinal tract. When comparing ESBL-producing and non-producing isolates, there was no difference (p>0.05) in the overall survival rate. The results demonstrate that, despite the decrease in microorganism counts during simulated passage through gastric and intestinal juices, the mean overall survival rate of Salmonella spp. isolates was high. This suggests that the isolates have the capacity to survive simulated gastrointestinal conditions and remain at levels capable of causing infection.

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