Perfil de susceptibilidade a antimicrobianos de cepas de Aspergillus sp. isolados de solo impactado

Sarah Vieira Guimarães; Geovanna da Silva Pinho Belém; Sthefany Lima Pire; Danyelle Cristina Pereira Santos; Suelem dos Santos Amaral Gouveia; Rita de Cássia de Miranda

doi:10.18593/evid.34486

Authors

Sarah Vieira Guimarães Uniceuma
Geovanna da Silva Pinho Belém Uniceuma
Sthefany Lima Pire Uniceuma https://orcid.org/0009-0008-4593-4065
Danyelle Cristina Pereira Santos Uniceuma https://orcid.org/0000-0002-8402-9913
Suelem dos Santos Amaral Gouveia Uniceuma
Rita de Cássia de Miranda UNIVERSIDADE CEUMA https://orcid.org/0000-0003-2116-1797

DOI:

https://doi.org/10.18593/evid.34486

Keywords:

Aspergillosis, Antifungal Agents, Microbial Sensitivity

Abstract

The composition of the landfill soil harbors a diversity of microorganisms that can become pathogenic to humans, such as those of the genus Aspergillus sp. This genus of fungi is characterized by the capacity for genetic alteration to increase resistance. Therefore, the aims of this work were to evaluate the resistance profile of fungi of the genus Aspergillus sp. isolated from impacted landfill soil in the city of Apicum-Açu, state of Maranhão, Brazil. For this purpose, strains of Aspergillus sp. (ASP 2, ASP3 and ASP 4), isolated from impacted soil, using the microculture technique to confirm the gender. The susceptibility of these microorganisms to antifungal agents was evaluated using the Minimum Inhibitory Concentration (MIC) technique by serial microdilution (1:2), in which the antifungals Amphotericin B (16 to 0.0312 mg/L), Voriconazole ( 16 to 0.0312 mg/L) and Itraconazole (8 to 0.0156 mg/L). Isolate ASP2 showed resistance to Voriconazole (>2 mg/L) and indefinite results for the antifungals Itraconazole and Amphotericin B. ASP3 demonstrated sensitivity to Itraconazole (0.5 mg/L) and Voriconazole (0.25 mg/L), however, the result was undefined for Amphotericin B. Finally, the ASP4 isolate showed resistance to Amphotericin B (2 mg/L) and sensitivity to Itraconazole (0.5 mg/L) and Voriconazole (1 mg/L). In this way, the increased resistance of Aspergillus sp. is evident, and the inefficiency of the degree of susceptibility of these drugs against pathologies, thus requiring more in-depth studies and research into new biomolecules, which will allow more efficient results against this genus. of fungi.

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References

ARENDRUP, M. C. et al. EUCAST technical note on Aspergillus and amphotericin B, itraconazole, and posaconazole. Clinical Microbiology and Infection, v. 18, n. 7, p. E248-E250, 2012.

BRANDL, J.; ANDERSEN, M. R. Aspergilli: Models for systems biology in filamentous fungi. Current Opinion in Systems Biology, v. 6, p. 67–73, 2017.

BRAGA, V. F. Determinação da prevalência das espécies, resistência a antifúngicos e perfil de virulência de Aspergillus spp. oriundos de amostras de origem clínica. 2018. Dissertação (Mestrado em Biociências Aplicadas à Farmácia) - Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, 2018.

BrCAST. Brazilian Committee on Antimicrobial Susceptibility Testing. Método para determinação de concentração inibitória mínima em caldo dos agentes antifúngicos para fungos filamentosos formadores de conídios. EUCAST – Documento definitivo E.DEF. 9.3.1, 2017.

DENARDI, L. B. et al. In vitro antifungal susceptibility of clinical and environmental isolates of Aspergillus fumigatus and Aspergillus flavus in Brazil. Brazilian jornal of Infectiuos Diseases, v. 22, n. 1, p. 30-36, 2018.

FIDELIS, R. R. et al. Indicadores biológicos de qualidade do solo em culturas intercalares ao pinhão manso. Brazilian Journal of Applied Technology for Agricultural Science, v. 9, n. 3, 87-95, 2016.

FREITAS, L. et al. Indicadores da qualidade química e física do solo sob diferentes sistemas de manejo. Unimar Ciências, v. 26, n. 1-2, p. 8-25, 2017.

GAVRONSKI, S. et al. Diagnóstico laboratorial de aspergilose invasiva: avaliação de métodos moleculares e detecção de antígenos. Revista Brasileira de Análises Clínicas, v. 48, n. 2, p. 96-109, 2016.

HOLT, J. G. et al. Bergey’s manual of systematic bacteriology. Baltimore: Williams & Wilkins, v. 4 p. 2300-2648, 1989.

HOPE, W. W. et al. EUCAST technical note on voriconazole and Aspergillus spp. Clinical Microbiology and Infection, v. 19, n. 6, p. E278-E280, 2013.

LEAL, M. L. A. et al. Efeito dos sistemas de manejo e do uso do solo na população de microrganismos do solo. Research, Society and Development, v. 10, n. 9, p. e21910917966-e21910917966, 2021.

MUNOZ-ROJAS M. et al. Soil physicochemical and microbiological indicators of short-, medium- and long-term post-fire recovery in semi-arid ecosystems. Ecological Indicators, v. 63, p. 14–22, 2016.

PONTES, L. Vigilância de resistência frente a antifúngicos azólicos de isolados clínicos de Aspergillus fumigatus. 2018. 1 recurso online (77 p.) Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas, Campinas, 2018.

QUINTANILHA-PEIXOTO, G. et al. Calm before the storm: A glimpse into the secondary metabolism of Aspergillus welwitschiae, the etiologic agent of the sisal bole rot. Toxins, v. 11, n. 11, p. 631, 2019.

REICHERT‐LIMA, F. et al. Surveillance for azoles resistance in Aspergillus spp. highlights a high number of amphotericin B‐resistant isolates. Mycoses, v. 61, n. 6, p. 360-365, 2018.

SALES, Maria da Penha Uchoa. Capítulo 5-Aspergilose: do diagnóstico ao tratamento. Jornal Brasileiro de Pneumologia, v. 35, p. 1238-1244, 2009.

SHIBUYA, Kazutoshi et al. Pathophysiology of pulmonary aspergillosis. Journal of Infection and Chemotherapy, v. 10, n. 3, p. 138-145, 2004.

SOUZA, D. R. et al. Efeitos tóxicos dos fungos nos alimentos. Revinter, v. 10, n. 2, p. 73-84, 2017.

VADLAPUDI, V. et al. Aspergillus Secondary Metabolite Database, a resource to understand the Secondary metabolome of Aspergillus genus. Scientific Reports, v. 7, no. 1, p. 1–10, 2017.