Concentrações de citocinina influenciam a multiplicação in vitro de kiwizeiro

Ana Luiza Arruda; Marceli Buss; Francine Regianini Nerbass; Leo Rufato

doi:10.18593/eba.v19i1.19723

Authors

Ana Luiza Arruda Universidade do Estado de Santa Catarina https://orcid.org/0000-0001-9791-4850
Marceli Buss Universidade do Estado de Santa Catarina https://orcid.org/0000-0003-1271-9306
Francine Regianini Nerbass Universidade do Estado de Santa Catarina https://orcid.org/0000-0002-6545-8771
Leo Rufato Universidade do Estado de Santa Catarina https://orcid.org/0000-0001-9545-7035

DOI:

https://doi.org/10.18593/eba.v19i1.19723

Keywords:

Micropropagation, Culture medium, Cytokinin

Abstract

The in vitro cultivation of kiwifruit in double-phase system appears as an alternative for improving the rate of multiplication of the species. Thus, the objective of this study was to evaluate the effect of different concentrations of BAP in the in vitro multiplication of kiwifruit in double-phase system. The experiment was carried out in the Laboratory of Plant Micropropagation belonging to the University of the state of Santa Catarina - Centro de Ciências Agroveterinárias (UDESC/CAV), in Lages. The treatments consisted of adding three concentrations of BAP (0,0; 1,0 and 2,0 mg L^-1) to the MS culture medium in a double-phase system. The variables analyzed after 30 days of cultivation of the in vitro explants were: number of shoots, average length of shoots, number of leaves and average length of leaves. The analysis of variance was significant for the linear regression and it was possible to observe a growth in the means of all variables analyzed as the BAP concentrations in the culture medium were increased. Thus, for in vitro multiplication of kiwifruit in a double-phase system the concentration of BAP to be used is 2 mg L^-1.

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References

Ferguson AR, Huang HW. Genetic resources of kiwifruit: domestication and breeding. Hortic Rev. 2007;(33):1-121.

Nishiyama I. Fruits of the Actinidia Genus. Adv Food Nutr Res. 2007;(52):293-324.

Silveira SV, Anzanello R, Simonetto PR, Gava R, Garrido LR, Santos RSS, Girardi CL. Aspectos técnicos da produção de kiwizeiro. Bento Gonçalves: Empresa Brasileira de Pesquisa Agropecuária; 2012. 79 p.

Vieira MJ, Argenta LC, Amarante CVT, Steffens CA, Vieira AMFD. Preservação da qualidade pós-colheita de kiwizeiro ‘Bruno’ pelo controle do etileno. Rev Bras Frutic. 2010;(32):397-406.

Disqual. Manual de boas práticas agrícolas: kiwizeiro. 2012. 25 p.

Oliveira MM, Fraser LG. Actinidia spp. In: Litz RE. Biotechnology of fruit and nut crops. 1a ed. Wallingford: CABI Pub.; 2005. p. 1-27.

Almeida NM, Pacheco Junior RG, Cézar JO, Gonçalves HÁ, Souza AS. Produção de mudas micropropagadas de mandioca (Manihot esculenta Crantz) em larga escala: uma inovação tecnológica. In: Congresso Brasileiro De Mandioca; Congresso Latino-Americano e Caribenho de Mandioca. Novembro 9-13, 2005. Foz do Iguaçu: SBM; 2015.

George EF. Plant tissue culture procedure: background. In: George EF, Hall MA, De Klerck GJ. Plant propagation by tissue culture. Dordrech: Springer; 2008. v.1.

Kadota M, Imizu K, Hirano T. Double-phase in vitro culture using sorbitol increases shoot proliferation and reduces hyperhydricity in Japanese pear. Sci Hortic. 2001;(89):207-215.

Garcia R, Pacheco G, Falcão E, Borges G, Mansur E. Influence of type of explant, plant growth regulators, salt composition of basal medium, and light on callogenesis and regeneration in Passiflora suberosa L. (Passifloraceae). Plant Cell Tiss Organ Cult. 2011;(106):47-54.

Akbas, FA, Isikalan C, Namli S, Basaran D. Micropropagation of kiwizeirofruit (Actinidia deliciosa). Int J Agric Biol. 2007;(1):489-493.

Murashige T, Skoog FA. Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962;(15):473-497.

Lucas MAK, Fagundes JD, Pereira DD, Sarmento MB. Micropropagação de violeta-africana (Saintpaulia ionantha Wendl.): efeito da benzilaminopurina na multiplicação. Ciênc Agrotec. 2007;(31):1380-1385.

Rubin S, Lima CSM, Bandeira JM, Ribeiro MV, Benitz LC, Peters JA, et al. Reguladores de crescimento na multiplicação in vitro de Thymus vulgaris L. Rev Bras Biocienc. 2007;(5):480-482.

Moncaleon P, Cañal MJ, Feito I, Rodriguez A, Fernandez B. Cytokinins and mineral nutrition in Actinidia deliciosa (kiwi) shoots cultured in vitro. J Pl Physiol. 1999;(155):606-612.

Torres AC, Caldas LS, Buso JA. Cultura de Tecidos e Transformação Genética de Plantas. Brasília, DF: EMBRAPA: CBAB; 1998. 509 p. v.1.

Grattaplaglia D, Machado MA. Micropropagação. In: TORRES AC, CALDAS LS, Buso JA. Cultura de tecidos e transformação genética de plantas. Brasília, DF: Embrapa – SPI: Embrapa – CNPH; 1998. p. 183-260.

Nachtigall JC, Zecca AGD, Figueiredo SLB, Fortes GRL. Influência da benzilaminopurina (bap) na multiplicação in vitro de kiwi (Actinidia deliciosa). Ciênc Rural. 1995;(25):23-26.

Fortes GRL, Santos Filho BG, Zecca AGD. Multiplicação in vitro de kiwi, cv. Hayward.

Rev Bras Frutic. 1992;(14):71-75.

Rodrigues DB, Nadal MC, Camargo SS, Assis AM, Schuch MW, Peil RMN, et al. Growth regulators and substrates for Oncidium baueri Lindl. Micropropagation. Semina. 2016;(37):2901-2910.

Benincasa MMP, LEITE IC. Fisiologia vegetal. Jaboticabal: Funep; 2002. 168 p.

Santos JA, Silva CR, Carvalho JG, Nascimento TB. Efeito do calcário dolomítico e nitrato de potássio no desenvolvimento inicial de mudas da bananeira ‘Prata-Anã’ (AAB), provenientes de cultura in vitro. Revista Brasileira de Fruticultura. 2004;(26):150-154.

Oliveira RP, Silveira DG, Silva SO. Concentração de BAP e a eficiência de micropropagação de bananeira tetraplóide (grupo AAAB). Scientia Agrícola. 2001;(58):73-78.