Toxicity of naphthalene in the Neotropical Fish Astyanax Lacustris (Characiformes: Characidae) and Geophagus Brasiliensis (Perciformes: Cichlidae)

Geonildo Rodrigo Disner; Sabrina Louise Moraes Calado; Helena Cristina Silva Assis; Marta Margarete Cestari

doi:10.18593/eba.v17i1.12976

Autores

Geonildo Rodrigo Disner Universidade Federal do Paraná
Sabrina Louise Moraes Calado Departamento de Farmacologia, Laboratório de Toxicologia Ambiental, Universidade Federal do Paraná (UFPR). Rua Francisco H. dos Santos 81531-990 Jardim das Américas. Curitiba PR Brasil.
Helena Cristina Silva Assis Departamento de Farmacologia, Laboratório de Toxicologia Ambiental, Universidade Federal do Paraná (UFPR). Rua Francisco H. dos Santos 81531-990 Jardim das Américas. Curitiba PR Brasil.
Marta Margarete Cestari Departamento de Genética, Laboratório de Mutagênese Ambiental, Universidade Federal do Paraná (UFPR). Rua Francisco H. dos Santos 81531-990 Jardim das Américas. Curitiba PR Brasil.

DOI:

https://doi.org/10.18593/eba.v17i1.12976

Palavras-chave:

Comet assay, Ecotoxicology, Fish, Genotoxicity, Hormesis.

Resumo

Polycyclic aromatic hydrocarbons are one of the most important organic pollutants in environmental studies. The aim of this study was to assess the naphthalene acute toxicity in two fish species, Astyanax lacustris (LLcust, 1875) and Geophagus brasiliensis (Quoy & Gaimard, 1824). The fish were exposed to naphthalene (0.005, 0.03, 0.3, and 3 mgL^-1) in water and after that the piscine micronucleus test in erythrocytes, comet assay in blood, liver and gill cells, glutathione S–transferase (GST) activity in the liver, and accumulation of naphthalene in the bile were performed. The susceptibility of the two species was similar and naphthalene was not genotoxic in all tested tissues. The liver GST activity may have been responsible for less damage observed in the liver while the highest DNA damage occurred in blood cells. However, low concentrations of naphthalene in water can stimulate apparent benefits, such as less DNA damage, which would be a compensatory response to an imbalance of homeostasis. The naphthalene is absorbed and can accumulate in the gall bladder, a greater accumulation of PAH was observed in A. lacustris, while G. brasiliensis did not differ from the control. The naphthalene concentrations are not genotoxic to the tested species, although they can potentially accumulate into the body.

Keywords: Comet assay. Ecotoxicology. Fish. Genotoxicity. Hormesis.

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Biografia do Autor

Geonildo Rodrigo Disner, Universidade Federal do Paraná

Biológo pela UNOESC, Mestre em Genética pela UFPR, doutorado em andamento no Laboratório de Mutagênese Ambiental, Departamento de Genética UFPR. Minhas experiências científicas compreendem: Metodologia da Pesquisa, Genética Geral, Genética Toxicológica, Mutagênese e Conservação Ambiental, Cultivo Celular, Saúde Ambiental, Recuperação de Áreas Degradadas, Filosofia da Ciência, e áreas relacionadas.

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