Enzymatic characterization of the lignocellulolytic secretome of Penicillium Variabile wild strain from post-milling sugarcane bagasse in submerged fermentation
DOI:
https://doi.org/10.18593/evid.36808Keywords:
glycosyl hydrolases, biochemical protein profile, submerged fermentation, enzymatic activities, Penicillium variabileAbstract
Given its availability of sugars, sugarcane bagasse is a highly favorable environment for colonization by microorganisms, including filamentous fungi that, in the fermentation process, produce efficient enzyme complexes and are among the best evaluated microorganisms for the production of enzymes industrial. In this sense, the present study aimed to evaluate the cellulolytic extract produced by Penicillium variabile isolated from post-crushed sugarcane bagasse. The crude enzymatic extract (CEE) was produced by submerged fermentation of the fungus, after partial purification and characterization of the enzymes (fungus supplied gently by BIOMM S/A). The enzymatic activities achieved were 8.772 U/mL of CMCase; 1.975 U/mL β-glucosidase; 0.069 U/mL of FPase; 1.610 U/mL of xylanase and 0.409 mg/mL of total protein (TP). The optimal pH for enzymatic activity of cellulases with Penicillium variabile was 4.8, and the stability of these enzymes stood at a pH range of 4.0-4.8. In relation to the thermostability, cellulases remained more stable at -20ºC. The SDS-PAGE profile of the CEE showed proteins in a molecular weight range of 12-100 kDa, and the activities of endoglucanase, β-glucosidase and xylanase were disclosed in the zymogram. The partial purification of CEE by size exclusion chromatography (Sephacryl® S-100) resulted in asymmetric peaks of 60 kDa and 90 kDa, which showed CMCase and β-glucosidase activities, respectively. Thus, our results open horizons for the establishment of biotechnological applications of Penicillium variabile, although more studies are needed to understand its physiology and regulatory mechanisms of enzyme production.
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