Selênio na germinação de sementes, no desenvolvimento e acúmulo de nutrientes em plântulas de pitaia in vitro (Hylocereus Costaricensis)

Sarah Dias Azevedo; Gabrielen de Maria Gomes Dias; Márcio Cleber de Medeiros Corrêa; Antônio Mateus Pinheiro

doi:10.18593/evid.34242

Authors

Sarah Dias Azevedo Universidade Federal do Ceará/Mestranda https://orcid.org/0000-0002-5105-1794
Gabrielen de Maria Gomes Dias Universidade Federal do Ceará/Professora Visitante
Márcio Cleber de Medeiros Corrêa Universidade Federal do Ceará/Docente
Antônio Mateus Pinheiro Universidade Federal do Ceará/Mestrando

DOI:

https://doi.org/10.18593/evid.34242

Keywords:

Selenate, Tisue culture, Pitaya

Abstract

The plant tissue culture technique can be an effective tool for propagating the pitai tree, since through it the seedlings are multiplied in large quantities, producing clones identical to the mother plant, free of pathogens and in a short period of time. The work was carried out with the objective of evaluating the germination of pitaya seeds (Hylocereus costaricensis) grown in vitro with added selenium and the development of resulting seedlings. Pital seeds (Hylocereus costaricensis) were used inoculated in MS medium added with sodium selenate (Na₂SeO₄) at concentrations 0.0; 28.0; 34.0; 40.0; 46.0 and 52.0 μmol L^-1, with 25 repetitions. Furthermore, 10 mg L^-1 of BAP and 0.1 mg L^-1 of NAA were added to the selenium treatments. The experiment was carried out in a completely randomized design and the tubes were kept in a growth room for 60 days. The parameters evaluated were: germination rate, seedling length, number of cladodes, number of roots and cladode length (cm). Element quantification was performed with inductively coupled plasma (ICPMS). Data were evaluated using polynomial regression curves. All statistical analyzes were performed using the R Studio software. The inclusion of sodium selenate in the culture medium provided an improvement in the germination rate, number of cladodes, cladode length and seedling length of H. costaricensis. However, with the exception of the number of cladodes, all other phylotechnical parameters evaluated appeared to have better development with estimated concentrations lower than 28 µmol L^-1 (lowest concentration used), indicating that at lower doses the plants would have better development. Furthermore, the availability of selenium in the culture medium was proportional to its absorption in H. costaricensis seedlings and the addition of Se in the MS medium affected nutrient absorption by H. costaricensis.

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