Esterification of acetic acid with alcohol isoamyl in the presence of enzymatic catalyst

Nádia Ligianara Nyari; Alessandro Rogerio Paulazzi; Raquel Vera Zamadei; Jamile Zeni; Rogério Marcos Dallago

doi:10.18593/eba.v19i2.20116

Autores

Nádia Ligianara Nyari Universidade Regional Integrada do Alto Uruguai e das Missões – URI – Erechim, RS, Brasil
Alessandro Rogerio Paulazzi Universidade Regional Integrada do Alto Uruguai e das Missões – URI - Erechim, RS, Brazil.
Raquel Vera Zamadei Universidade Regional Integrada do Alto Uruguai e das Missões – URI - Erechim, RS, Brazil
Jamile Zeni Universidade Regional Integrada do Alto Uruguai e das Missões – URI - Erechim, RS, Brazil
Rogério Marcos Dallago Universidade Regional Integrada do Alto Uruguai e das Missões – URI - Erechim, RS, Brazil

DOI:

https://doi.org/10.18593/eba.v19i2.20116

Palavras-chave:

Candida antarctica Lipase B, Síntese de éster, Acetato de isoamila, Biocatálise

Resumo

A Lipase B da Candida antarctica imobilizada em esterificação catalisada por poliuretano de ácido acético com álcool isoamílico em sistema mecânico e ultrassônico em sistema isento de solventes foi estudada. A esterificação máxima do acetato de isoamila catalisador imobilizado utilizado foi a maior esterificação, 666,05 U/g com 19 ciclos de reuso após 360 minutos de tempo de reação no sistema mecânico e 1319,48 U/g foi observado, e 14 ciclos de reuso após 60 minutos de reação no sistema de ultrassom. Assim, o processo foi considerado eficiente com redução significativa do tempo de reação, baixos requisitos instrumentais e melhora no desempenho dos bioprocessos. Até o momento não havia estudos disponíveis na literatura aberta com relação à síntese de ésteres catalisada pela Lipase imobilizada em poliuretano como suporte no sistema de ultrassom. Além disso, considerada uma tecnologia ambientalmente correta e economicamente viável, pode ser usada em cosméticos, produtos farmacêuticos e indústria alimentícia.

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